Figure 1: Analysis of subcellular protein fractions of P50 midgut by SDS-PAGE.

1, markers; 2, total proteins; 3, mitochondrial proteins; 4, microsomal proteins; 5, cytosolic proteins. A quantity of 35 μg of each protein sample was loaded and electrophoresed in a 12% polyacrylamide gel and stained with Coomassie brilliant blue R250. Arrows represented differential and the same bands in the subcellular fractions. The full-length gels were included in the Supplementary Fig. S1.