Table 2 Identified proteins from cytosolic fraction that changed significantly in different resistant strains following BmNPV infection.

From: Comparative Subcellular Proteomics Analysis of Susceptible and Near-isogenic Resistant Bombyx mori (Lepidoptera) Larval Midgut Response to BmNPV infection

Spot no. a

P50+ vs. P50− b

Ratio b

Accession no. c

Protein name c

Theoretical/Observed PId

Theoretical/Observed MW (kDa) d

Matched unique peptides e

Sequence coverage (%) e

Protein score e

Molecular/biological function f

c1

down

49.13

gi|255652881

Dnaj (Hsp40) homologue 3

5.56/6.47

40/35.25

6

23%

456

Protein folding

c2

down

65.71

gi|512914963

Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial isoform X1

7.59/7.53

56/57

9

22%

808

Aldehyde dehydrogenase (NAD) activity, fatty-acyl-CoA binding, methylmalonate semialdehyde dehydrogenase (acylating) activity, thymine metabolic process, valine metabolic process

c3

down

149.67

gi|512936895

Acetyl-CoA hydrolase

7.67/7.83

52/48.4

8

16%

523

Hydrolase activity, acetyl-CoA metabolic process

c4

down

6.07

gi|512902782

Uncharacterized protein LOC101738880 isoform X1

5.75/5.72

25/22.73

7

34%

578

 

c5

down

5.34

gi|17136564

Alpha-tubulin at 84B [Drosophila melanogaster]

5.00/5.62

51/53.44

8

27%

767

GTPase activity, GTP binding, structural constituent of cytoskeleton, antimicrobial humoral response, mitotic spindle assembly checkpoint

c6

down

2.4

gi|512934077

10 kDa heat shock protein, mitochondrial

6.74/7.38

11/15.22

4

52%

396

ATP binding, protein folding

c7

down

2.86

gi|827563568

Electron transfer flavoprotein subunit alpha, mitochondrial

8.43/7.2

35/29.68

8

36%

827

Electron carrier activity, flavin adenine dinucleotide binding

c8

down

3.72

gi|827558088

3-hydroxyisobutyryl-CoA hydrolase, mitochondrial

8.08/7.43

41/35.39

7

27%

584

Hydrolase activity

c9

down

8.15

gi|512898603

Glyoxylate reductase/hydroxypyruvate reductase-like isoform X1

8.76/7.65

40/36.95

10

33%

849

NAD binding, oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

c10

down

148.54

gi|112984390

Elongation factor 1-alpha

9.24/6.69

51/49.5

6

18%

390

GTPase activity, GTP binding, translation elongation factor activity

c11

up

3.09

gi|112983556

90-kDa heat shock protein

4.99/5.52

83/86.37

9

16%

713

ATP binding, response to stress, protein folding

c12

up

5.75

gi|512901366

Aldose reductase-like isoform X1

6.09/6.68

36/31.36

8

30%

491

Oxidoreductase activity

c13

up

4.58

gi|827560339

Prolyl endopeptidase

7.90/6.61

90/76.05

4

6%

309

Serine-type endopeptidase activity, serine-type exopeptidase activity

c14

up

3.66

gi|512888904

Cytoplasmic aconitate hydratase-like isoform X1

5.84/6.73

97/94.81

8

13%

55

Metabolic process

c15

up

5.93

gi|512939991

Cystathionine beta-synthase-like

6.02/6.86

54/54.31

8

18%

481

Cystathionine beta-synthase activity, metal ion binding, pyridoxal phosphate binding

c16

up

3.12

gi|357613322

26 S protease regulatory subunit 6 A [Danaus plexippus]

5.11/5.73

48/49.32

7

23%

493

ATP binding, peptidase activity, protein catabolic process

c17

up

3.14

gi|312597598

Inorganic pyrophosphatase

4.96/5.4

32/29.85

9

28%

452

Inorganic diphosphatase activity, magnesium ion binding, phosphate-containing compound metabolic process

c18

up

6.1

gi|512923641

Fatty acid-binding protein-like

5.04/6.16

16/18.89

5

38%

296

Lipid binding, transporter activity

c19

up

4

gi|512923641

Fatty acid-binding protein-like

5.04/6.7

16/16.44

5

33%

200

Lipid binding, transporter activity

c20

up

8.06

gi|512917297

Fatty acid-binding protein 1-like isoform X1

6.59/6.67

15/15.61

7

71%

573

Lipid binding, transporter activity

Spot no. a

BC9− vs. P50− b

Ratio b

Accession no. c

Protein name c

Theoretical/Observed P I d

Theoretical/Observed MW (kDa) d

Matched unique peptides e

Sequence coverage (%) e

Protein score e

Molecular/biological function f

c21

down

4.29

gi|512907055

Grpe protein homologue, mitochondrial

6.97/6.2

24/21.21

6

44%

491

Adenyl-nucleotide exchange factor activity, protein folding

c23

down

2.13

gi|114051229

Microtubule-associated protein RP/EB family member 3

5.48/6.27

31/29.98

9

37%

829

 

c24

down

2.15

gi|291045214

Isopentenyl-diphosphate delta isomerase

6.37/6.57

30/27.16

8

31%

325

Hydrolase isopentenyl-diphosphate delta-isomerase activity, isoprenoid biosynthetic process

c25

down

2.53

gi|512892238

Carbonic anhydrase 2

5.92/6.41

31/27.45

5

28%

420

Carbonate dehydratase activity, one-carbon metabolic process

c26

down

2.32

gi|160333678

Glutathione S-transferase sigma 2

5.85/6.89

23/24.17

9

53%

864

Transferase activity

c27

down

4.37

gi|112982671

Ribosomal protein S12

5.79/6.21

15/15.97

6

75%

385

Structural constituent of ribosome, translation

c28

up

64.59

gi|49868

Beta-actin (aa 27–375) [Mus musculus]

5.78/6.13

39/32.55

5

19%

389

ATP binding, identical protein binding, kinesin binding, nitric-oxide-synthase binding, RNA polymerase II core promoter proximal region sequence-specific DNA binding

c29

up

72.24

gi|114051866

Isocitrate dehydrogenase

6.24/6.91

47/43.92

7

15%

329

Isocitrate dehydrogenase (NADP+) activity, magnesium ion binding, NAD binding, isocitrate metabolic process, tricarboxylic acid cycle

c30

up

2.96

gi|153792114

Phosphatidylethanolamine-binding protein isoform 2

5.96/5.81

22/20.57

5

39%

230

Defence response to Gram-negative/positive bacteria, regulation of antimicrobial humoral response

c31

up

9.61

gi|512902782

Uncharacterized protein LOC101738880 isoform X1

5.75/5.55

25/20.54

8

45%

791

 

c32

up

4.1

gi|4574740

Tat-binding protein-1 [Drosophila melanogaster]

5.39/5.73

48.4/49.32

3

13%

277

ATPase activity, ATP binding, proteasome-activating ATPase activity, TBP-class protein binding

c33

up

4.88

gi|51555848

Glycerol-3-phosphate dehydrogenase-2

5.62/6.4

39/31.69

10

34%

850

Glycerol-3-phosphate dehydrogenase [NAD+] activity, NAD binding, carbohydrate metabolic process, glycerol-3-phosphate catabolic process

c34

up

2.53

gi|114053311

26 S protease regulatory subunit 6B

5.09/5.61

47/50.98

6

17%

337

ATP binding, peptidase activity, protein catabolic process

c35

up

58.24

gi|347326520

DNA supercoiling factor

4.48/5.53

40/40.96

7

27%

476

Calcium ion binding

Spot no. a

BC9+ vs. BC9− b

Ratio b

Accession no. c

Protein name c

Theoretical/Observed P I d

Theoretical/Observed MW (kDa) d

Matched unique peptides e

Sequence coverage (%) e

Protein score e

Molecular/biological function f

c36

down

2.79

gi|512891246

Proteasome subunit alpha type-1 isoform X2

6.01/6.82

31/31.02

8

39%

516

Endopeptidase activity, threonine-type endopeptidase activity

c37

up

136.6

gi|512934137

Selenium-binding protein 1 isoform X2

5.68/6.42

53/52.96

5

15%

298

Selenium binding

c38

up

4.9

gi|512914963

Probable methylmalonate-semialdehyde dehydrogenase [acylating]

7.59/7.36

56/54.89

10

23%

758

Aldehyde dehydrogenase (NAD) activity, fatty-acyl-CoA binding, methylmalonate semialdehyde dehydrogenase (acylating) activity, thymine metabolic process, valine metabolic process

c39

up

10.69

gi|156255210

L-lactate dehydrogenase

6.76/7.72

37/33.93

3

9%

172

L-lactate dehydrogenase activity, carbohydrate metabolic process, carboxylic acid metabolic process

  1. aSpot no. corresponding to the numbers in the 2-DE gels of Fig. 2.
  2. bThe expression intensity ratios of P50 and BC9 following BmNPV infection or strain BC9 to that of P50.
  3. cDetailed information on the identified proteins can be viewed via their accession numbers on http://www.ncbi.nlm.nih.gov/.ncbi.nlm.nih.gov/.
  4. dObserved molecular weight (MW) and isoelectric point (PI) values were obtained from PDQuest analysis. Theoretical MW and PI were obtained from a MASCOT analysis.
  5. eThe three parameters, matched unique peptides, sequence coverage and protein score were generated from a MASCOT analysis.
  6. fMolecular/biological functions were annotated using the gene ontology (GO) database (http://www.geneontology.org/).
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