Table 3 Identified proteins from mitochondrial fraction that changed significantly in different resistant strains following BmNPV infection.
Spot no.a | P50+ vs. P50−b | Ratiob | Accession noc | Protein namec | Theoretical/Observed PId | Theoretical/Observed MW (kDa)d | Pep. Counte | Sequence coverage (%)e | Protein Scoree | Molecular/biological functionf |
|---|---|---|---|---|---|---|---|---|---|---|
mc1 | down | 4.23 | gi|62241292 | Protein disulfide-isomerase | 5.3/5.75 | 55.5/52.39 | 17 | 99.97 | Protein disulfide isomerase activity, cell redox homeostasis | |
mc2 | down | 11.58 | gi|336454478 | Heat shock protein 70–3 | 5.12/6.9 | 72.8/30.53 | 17 | 100 | ATP binding | |
mc3 | down | 6.31 | gi|827547570 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex | 9.28/6.93 | 50.4/53.97 | 7 | 18% | 361/58 | Dihydrolipoyllysine-residue succinyltransferase activity, tricarboxylic acid cycle |
mc4 | up | 12.41 | gi|114052454 | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 | 5.93/6.62 | 19.3/17.96 | 8 | 60% | 521/58 | Oxidation-reduction process |
mc5 | up | 17.81 | gi|512890394 | Golgin subfamily A member 4 | 5.05/6.78 | 30.8/17.3 | 43 | 95.118 | Protein targeting to Golgi | |
mc6 | up | 11.56 | gi|512928976 | Voltage-dependent anion-selective channel isoform X2 | 6.96/6.79 | 30.1/27.03 | 18 | 100 | Voltage-gated anion channel activity | |
mc7 | up | 3.65 | gi|512928976 | Voltage-dependent anion-selective channel isoform X2 | 6.96/7.42 | 30.1/27.03 | 17 | 100 | Voltage-gated anion channel activity | |
mc8 | up | 5.02 | gi|98990259 | Cytochrome b-c1 complex subunit Rieske | 8.59/7.25 | 29.4/21.47 | 11 | 99.982 | 2 iron, 2 sulfur cluster binding, metal ion binding, ubiquinol-cytochrome-c reductase activity | |
Spot no. a | BC9− vs. P50− b | Ratio b | Accession no. c | Protein name c | Theoretical/Observed P I d | Theoretical/Observed MW (kDa) d | Matched unique peptides e | Pep. Count e | PROTEIN score(%) e | Molecular/biological function f |
mc9 | up | 2.58 | gi|87248369 | NADH dehydrogenase (ubiquinone) Fe-S protein 8 | 6.15/5.79 | 25.5/21.83 | 13 | 100 | 4 iron, 4 sulfur cluster binding, oxidoreductase activity, acting on NAD(P)H | |
mc10 | up | 3.86 | gi|38260562 | Thiol peroxiredoxin | 6.09/7 | 22.07/19.63 | 10 | 100 | Peroxiredoxin activity | |
mc11 | up | 3.62 | gi|98990259 | Cytochrome b-c1 complex subunit Rieske | 8.59/7.25 | 29.4/21.47 | 11 | 99.982 | 2 iron, 2 sulfur cluster binding, metal ion binding, ubiquinol cytochrome c reductase activity | |
Spot no. a | BC9+ vs. BC9− b | Ratio b | Accession no. c | Protein name c | Theoretical/Observed P I d | Theoretical/Observed MW (kDa) d | Matched unique peptides e | Pep. Count e | Protein score(%) e | Molecular/biological function f |
mc12 | down | 6.05 | gi|95102940 | H+-transporting ATP synthase beta subunit isoform 2 | 5.32/5.92 | 54.9/47 | 15 | 99.488 | ATP binding, proton-transporting ATP synthase activity | |
mc13 | down | 4.44 | gi|87248085 | Chaperonin-containing t-complex polypeptide 1 beta | 6.32/6.96 | 58/55.19 | 18 | 100 | ATP binding, protein folding | |
mc14 | up | 2.1 | gi|512892238 | Carbonic anhydrase 2 | 5.92/6.31 | 30.04/26.15 | 9 | 100 | Carbonate dehydratase activity, one-carbon metabolic process |
