Figure 5: Intranigral infusion of acrolein induced programmed cell death in the nigrostriatal dopaminergic system of rat brain. | Scientific Reports

Figure 5: Intranigral infusion of acrolein induced programmed cell death in the nigrostriatal dopaminergic system of rat brain.

From: Acrolein acts as a neurotoxin in the nigrostriatal dopaminergic system of rat: involvement of α-synuclein aggregation and programmed cell death

Figure 5

(A and C), For a dose-dependent effect, ARC (15–150 nmoles) was locally infused in the substantia nigra (SN) and rats were sacrificed 7 days after intranigral infusion of acrolein. (B and D): For a time-dependent effect, acrolein (150 nmoles) was locally infused in the SN and rats were sacrificed at the time indicated. Western blot assay was employed to detect caspase 3 activation (A,B) as well as RIP-1 and RIP-3 (C,D) in the SN. Each lane contained 25 μg protein for all experiments. Graphs show statistic results from relative optical density of bands on the blots estimated by Image J. Values are the mean ± S.E.M. (n = 5/group for A, n = 3/group for B–D). *p < 0.05 in the acrolein-infused SN compared with the intact SN by one-way analysis of variance (one-way ANOVA) and followed by the LSD test as a post-hoc analysis.

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