Figure 4: Hierarchical cluster analysis of differentially expressed genes (DEGs) in cold- and TDZ-treated plants.

Each column represents an experimental sample (CK-control, LT-cold treatment, and TDZ-TDZ treatment), and each row represents a gene. 54 DEGs that were significantly regulated (p-value < 0.05) were analyzed in pairwise comparisons (CK-VS-LT, CK-VS-TDZ,and LT-VS-TDZ). Expression levels were determined by RPKM (Reads Per kb per Million reads) values calculated from Illumina read counts, according to the method provided by Mortazavi and Williams (2008). Expression differences are shown in different colors. Red indicates high expression, and green indicates low expression.