Figure 4: Suppression of proliferation of mesothelioma cells by HEG1 siRNA. | Scientific Reports

Figure 4: Suppression of proliferation of mesothelioma cells by HEG1 siRNA.

From: HEG1 is a novel mucin-like membrane protein that serves as a diagnostic and therapeutic target for malignant mesothelioma

Figure 4

Values are means ± S.D. of tetraplicate determinations. (a) Time-course of cell growth of ACC-MESO-4 treated with HEG1 siRNA. siRNA was transfected with Lipofectamine 2000. HEG1 siRNA mix 1, a mixture of H1097 and H2674 (1:1); HEG1 siRNA mix 2, sc-78365 (Santa Cruz Biotechnology); Control siRNA, MISSION siRNA Universal Negative Control (SIC-001). Similar results were obtained in 2 independent experiments. (b) Suppression of cell proliferation of ACC-MESO-4 with several HEG1 siRNAs. The cells were cultured for 72 h with siRNA and Lipofectamine 2000. Control siRNA, MISSION siRNA Universal Negative Control. Similar results were obtained in 2 independent experiments. (c) Suppression of cell proliferation with HEG1 siRNA on MPM cell lines. The cells were treated for 72 h with siRNA and Lipofectamine RNAiMAX. HEG1 expression after 72 h was shown in western blotting using mAb SKM9-2 at the bottom of the figure. Differences between mean values were analyzed by Student’s t-test. P-values for tests of ACC-MESO-4 and NCI-H2452 were <0.01. Differences for other cells were not significant. Control was treated with a control siRNA, MISSION siRNA Universal Negative Control; HEG1 was treated with a HEG1 siRNA, H2674. Similar results were obtained in 2 independent experiments.

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