Figure 5: Distribution of GPR91 in the retinal ganglion cells.
From: Transcription factors regulate GPR91-mediated expression of VEGF in hypoxia-induced retinopathy
(A) Western blot analysis of GPR91 protein expression in retinal ganglion cells treated with CoCl2 for 24 h. (B) Quantitative analysis of band density. Each column denotes the mean ± SD (n = 3). (C) Immunofluorescence showing GPR91 expression in the cytoplasm of retinal ganglion cells. Green fluorescence shows the distribution of the GPR91 protein. Red fluorescence shows the distribution of the retinal ganglion cell marker Thy1.1 protein. Blue fluorescence shows nuclei stained with 4,6-diamidino-2-phenylindole (DAPI). The right column shows the merged pictures. (D) Changes in mRNA levels (as determined using qRT-PCR) of GPR91 in samples from each group. (E) Western blot analysis of the GPR91 protein in samples from each group. (F) Quantitative analysis of the band density of GPR91/β-actin. Each column denotes the mean ± SD (n = 3). **P < 0.01 versus the NC 72 h group.
