Figure 9: GPR91 modulated the CoCl2-induced increase in ERK1/2 signaling, C/EBP β, c-Fos, HIF-1α activity and VEGF expression in retinal ganglion cells.
From: Transcription factors regulate GPR91-mediated expression of VEGF in hypoxia-induced retinopathy
(A) Changes in ERK1/2 phosphorylation (using western blot) in retinal ganglion cells transduced with LV.shScrambled or LV. shGPR91. (B) Quantitative analysis of band density. Each column denotes the mean ± SD (n = 3). (C) qRT-PCR analysis for C/EBP β and c-Fos mRNA in retinal ganglion cells from each group. (D) Western blot analysis of C/EBP β, c-Fos and HIF-1α protein expression in retinal ganglion cells treated with CoCl2 for 24 h. The cells were transduced with GPR91 siRNA or pre-treated with U0126 (ERK1/2 inhibitor). (E) Quantitative analysis of band density. (F) Changes in mRNA levels of HIF-1αVEGF in samples from each group. (G) ELISA analysis of VEGF secretion in samples from each group. (H) Western blot analysis of VEGF secretion in samples from each group. (I) Quantitative analysis of band density. Each column denotes the mean ± SD (n = 3). Each column denotes the mean ± SD (n = 3). **P < 0.01 versus control. ##P < 0.01 versus NC siRNA group. ψψP < 0.01 versus CoCl2 group.
