Figure 1: Quantification of plant growth.
To quantify growth within a plate using ImageJ, an original image (i) was filtered using the ‘Threshold Colour’ plug-in so that only the shades of green are retained (ii) before the image was converted to an 8-bit image (iii) and threshold adjusted to convert grey shades into red pixels that are measurable by ImageJ (iv). In this way, from an image, the green pixels can be counted and a value for total area in mm2 obtained.
