Figure 1: Interaction of H3K9ac-mintbody-GFP with acetylated H3K9 in tobacco BY-2 cells.

(a) H3K9ac levels in response to TSA treatments in BY-2 cells. The upper panel shows immunoblotting of H3K9ac and H3. The lower panel shows the quantification of H3K9ac levels calculated from the intensity of each band. H3K9ac levels were normalized with H3 levels. The values represent means ± S.D.. Distinct letters on the bars indicate significant difference at each treatment time (n = 3, Tukey’s multiple range test: p < 0.05). For the full-length western blots see Supplementary Fig. S4a. (b) Analysis of co-immunoprecipitation of endogenous H3K9ac with H3K9ac-mintbody-GFP in H3K9ac-mintbody-GFP expressing tobacco BY-2 cells treated with 1 μM TSA for 3 h. For the full-length western blots see Supplementary Fig. S4b. (c) Analysis of co-immunoprecipitation of endogenous H3K9me2 with H3K9ac-mintbody-GFP in H3K9ac-mintbody-GFP expressing tobacco BY-2 cells treated with 10 μM C646 for 3 h. For the full-length western blots see Supplementary Fig. S4c.