Figure 3

Bacterial depletion with deployable micro-traps, from experiments (a–c) and numerical simulations (d–f). (a) Red triangles: number of E. coli bacteria per μL present in a tube with 30 micro-traps (Fp) per μL as a function of time. Blue dots: control experiment without micro-traps. (b) Percentage of bacteria left in the suspending medium as a function of time, in the presence of 30 micro-traps (Fp) per μL. Error bars correspond to two independent experiments. (c) Percentage of bacteria left in the suspending medium after 180 minutes. Blue bar (-): tube without micro-traps. Violet bar (Cp): tube with micro-traps having straight apertures, concentration 30 per μL (4 independent experiments). First red bar (Fp): tube with micro-traps having funnel apertures, concentration 30 per μL (5 independent experiments). Second red bar (2 × Fp): tube with micro-traps having funnel apertures, concentration 60 per μL. (d) Simulated bacterial distribution in the case of a micro-trap with funnel apertures and 2 layers (Fp). (e) Time course of the accumulation obtained from numerical simulations of micro-traps with 2 internal layers (Cp, Fp), 3 internal layers (3Lp), and 5 internal layers (5Lp). (f) Maximum depletion reached for simulated micro-traps with 2 internal layers (Cp, Fp), 3 internal layers (3Lp), and 5 internal layers (5Lp).