Figure 10: Scheme of the treatments and harvest for expression analysis of PnAlaAT genes.

(a), distribution of different organs in P. simonii × P. nigra, leaves: 1st–3rd (L1) and 4th–6th (L2) are the ones from the top of the plants, 1st-3rd (L3) are the ones from the bottom of the plants. (b), the four factors (tissue, N source, concentration and time). The control was treated with nitrogen-free nutrient solution for 0 h. There were 420 samples in total.