Figure 3
DFS suppresses β-catenin nuclear translocation by reducing the level of FoxM1 protein. SW480 and HCT116 colon cancer cells were treated with the indicated concentrations of DFS for 16 h and the protein levels of β-catenin and its target genes were measured (A). The cytosolic and nuclear protein levels of β-catenin were determined by Western blot (B). SW480 cells were transfected with siRNA against FoxM1 and the localization of β-catenin was determined by Western blot (C,D). SW480 and HCT116 colon cancer cells were treated with the indicated concentrations of DFS for 16 h, and the protein and mRNA levels of FoxM1 were determined by Western blot and RT-PCR (E).
