Figure 4: DFS induces lysosomal-dependent FoxM1 protein degradation. | Scientific Reports

Figure 4: DFS induces lysosomal-dependent FoxM1 protein degradation.

From: A lignan induces lysosomal dependent degradation of FoxM1 protein to suppress β-catenin nuclear translocation

Figure 4

SW480 and HCT116 colon cancer cells were treated with DFS and cycloheximide (CHX) for 14 h and the protein levels of FoxM1 were determined by Western blot (A). HCT116 colon cancer cells were infected with adeno-FoxM1 virus and treated with DFS and CHX for 14 h, then the protein levels of FoxM1 were determined by Western blot (B). SW480 and HCT116 colon cancer cells were treated with DFS and Etoposide (ET) for 16 h and the protein and mRNA levels of FoxM1 were determined by Western blot and RT-PCR (C). SW480 and HCT116 colon cancer cells were treated with DFS and MG132 for 16 h and the protein and mRNA levels of FoxM1 were determined by Western blot and RT-PCR. Then, the membrane was reprobed by stripping buffer and HIF1α protein levels were measured to assess MG132 activity (D). SW480 cells were transfected with the indicated siRNAs and related gene expressions were determined by RT-PCR and Western blot assay (E). SiRNA-transfected SW480 cells were treated with DFS for 16 h and FoxM1 protein levels were determined by Western blot (F). Black triangle denotes original FoxM1 and the brace denotes post-modified FoxM1.

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