Figure 2: Antigen specific release of IFN-γ in patients (left, n = 74) and controls (right, n = 100) stimulated with QFT and ESAT-6 free IGRA cocktails. | Scientific Reports

Figure 2: Antigen specific release of IFN-γ in patients (left, n = 74) and controls (right, n = 100) stimulated with QFT and ESAT-6 free IGRA cocktails.

From: Introducing the ESAT-6 free IGRA, a companion diagnostic for TB vaccines based on ESAT-6

Figure 2

One ml whole blood was stimulated 18–24 h in antigen coated heparinized vacutainer tubes (in-house and QFT (Qiagen)), antigen specific IFN-γ release was determined using QFT ELISA (Qiagen) and is presented subtracting unspecific release (nil) in heparinized vacutainer tubes with no peptides (in-house and QFT (Qiagen)). Kruskal-Wallis test.

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