Table 1 Primary antibodies used in this study.
Antibody | Phosphorylation sites | Source |
|---|---|---|
pS199/202 | Ser199/202 | Invitrogen, Grand Island, NY |
pT205 | Thr205 | Invitrogen |
pS262 | Ser262 | Invitrogen |
pS396 | Ser396 | Invitrogen |
Tau-5 | Millipore, Temecula, CA, USA | |
GSK-3α/β | Cell Signaling Technology, Danvers, MA | |
GSK-3α/β pS21/9 | Ser21/9 | Cell Signaling Technology |
CDK5 | Santa Cruz Biotechnology, Santa Cruz, CA | |
P35 | Cell Signaling Technology | |
ERK1/2 | Cell Signaling Technology | |
ERK1/2(pT202/pY204) | Thr202/Tyr204 | Cell Signaling Technology |
JNK | Cell Signaling Technology | |
JNK(pT183/pY185) | Thr183/Tyr185 | Cell Signaling Technology |
CaMKII | Santa Cruz Biotechnology | |
CaMKII (pT286) | Thr286 | Santa Cruz Biotechnology |
Methyl-PP2A-C | Millipore | |
PP2A(pY307) | Tyr307 | Santa Cruz Biotechnology |
PP2A-C | Millipore | |
GFAP | Millipore | |
Iba1 | Wako Chemicals, Richmond, VA, USA | |
Doublecortin | Santa Cruz Biotechnology | |
Synapsin 1 | Santa Cruz Biotechnology | |
Synaptophysin | Millipore | |
PSD95 | Cell Signaling Technology | |
IRβ | Cell Signaling Technology | |
p-IRβ/IGF-1Rβ | Tyr1150/1151(IRβ), Tyr1135/1136 (IGF-1Rβ) | Cell Signaling Technology |
IRS1 | Cell Signaling Technology | |
IRS1 pS636 | Ser636 | Santa Cruz Biotechnology, Santa Cruz, CA |
PI3K p85 | Cell Signaling Technology | |
p-PI3K p85 | Tyr458 | Cell Signaling Technology |
PDK1 | Cell Signaling Technology | |
PDK1 pS241 | Ser241 | Cell Signaling Technology |
AKT | Cell Signaling Technology | |
AKT pT308 | Thr308 | Cell Signaling Technology |
AKT pS473 | Ser473 | Cell Signaling Technology |
GAPDH | Santa Cruz Biotechnology |
