Figure 2: Sensitivity for external stressors. | Scientific Reports

Figure 2: Sensitivity for external stressors.

From: Non-photonic sensing of membrane-delimited reactive species with a Na+ channel protein containing selenocysteine

Figure 2

(a) Mean time courses of non-inactivating current fraction for the indicated constructs with the application of 1 μM chloramine T (ChT). (b) Time courses of the F400/F470 fluorescence ratios of roGFP2 expressed in HEK 293 cells with extracellular application of ChT. (c) Time constants of ChT-induced loss of inactivation. The green circle indicates that 3 μM ChT had a negligible effect on the ratiometric roGFP2 signal. (d) Mean time courses of non-inactivating current fraction for the indicated constructs with the application of 500 μM H2O2 (at 32 °C). (e) Fluorescence ratios of roGFP2 as in (b) for the indicated H2O2 applications. (f) Time constants of H2O2-induced loss of inactivation (IFC, IFU) and changes in fluorescence ratio (roGFP2). Data are mean ± s.e.m. with n indicated in parentheses. ***P < 0.001; **P < 0.01 for a two-sided t-test; in (c) IFU vs. IFC; in (f) IFU vs. roGFP2.

Back to article page