Figure 1: Rb and DNMT3b bind to different regions in FoxM1b. | Scientific Reports

Figure 1: Rb and DNMT3b bind to different regions in FoxM1b.

From: Plk1 Regulates the Repressor Function of FoxM1b by inhibiting its Interaction with the Retinoblastoma Protein

Figure 1

MCF7 cells were transiently transfected with GFP-Control, GFP-FoxM1 full length (FL), GFP-FoxM1 mutant (232–748) or a GFP fusion construct containing the N-terminal 331 residues of FoxM1. 48hr post transfection, lysates (800 ug) were immunoprecipitated with GFP antibody and fractionated by SDS PAGE. Western blot analysis was performed with a monoclonal antibody specific for Rb (A) or antibody against DNMT3b (C). Immunoprecipitations of the GFP-fusion proteins are shown in panel B, and expression of GFP constructs in experiment C are shown in panel D.

Back to article page