Figure 2: Rb binding site overlaps with the Plk1 phosphorylation sites in FoxM1b. | Scientific Reports

Figure 2: Rb binding site overlaps with the Plk1 phosphorylation sites in FoxM1b.

From: Plk1 Regulates the Repressor Function of FoxM1b by inhibiting its Interaction with the Retinoblastoma Protein

Figure 2

MCF7 cells were transiently transfected with GFP-FoxM1 (FL) or the indicated FoxM1 deletion mutants. Cell lysates were prepared from transfected cells after 48 hr. Cell lysates (700 ug) were immunoprecipitated with GFP antibody followed by western blotting with Rb-antibody (A,C). Immunoprecipitation of the GFP constructs are shown in panels B and D. (E) Schematic representation of the Rb binding site within the C-terminal domain of FoxM1.

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