Figure 5: A Plk1-site phospho-mimetic mutant of FoxM1b fails to bind Rb in vitro. | Scientific Reports

Figure 5: A Plk1-site phospho-mimetic mutant of FoxM1b fails to bind Rb in vitro.

From: Plk1 Regulates the Repressor Function of FoxM1b by inhibiting its Interaction with the Retinoblastoma Protein

Figure 5

T7-His tagged C-terminal FoxM1 (residues 508–748), Plk1-site phospho-mimetic DD mutant, and GST-Rb (residues 379–928) were all expressed separately in E. coli. The bacterial lysates of the wild type or DD mutant FoxM1 were mixed with the lysates containing either GST-Rb or GST-CBP-KIX and then were allowed to bind Ni-agarose column. The eluted proteins, after extensive washing of the column, were assayed for the presence of Rb and CBP by western blotting (A and B). The left lane in each of the panels indicates the absence of Rb or CBP-KIX in the column elute when GST-Rb or CBP-KIX were passed through the Ni column in absence of FoxM1.

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