Figure 6: Plk1 phosphorylation regulates FoxM1b’s ability to methylate and repress GATA3 promoter. | Scientific Reports

Figure 6: Plk1 phosphorylation regulates FoxM1b’s ability to methylate and repress GATA3 promoter.

From: Plk1 Regulates the Repressor Function of FoxM1b by inhibiting its Interaction with the Retinoblastoma Protein

Figure 6

Cells were transfected with empty vector control, WT-T7-FoxM1, T7-FoxM1 (596 A), T7-FoxM1 (DD) and T7-FoxM1 (AA) expressing plasmids. Genomic DNA and RNA were extracted 48 h post transfection for GATA3 promoter methylation and transcripts quantification. (A). Lower panel shows the schematic of CpG island on GATA-3 promoter and primer design. In the upper panel, genomic-DNAs were isolated and C/T conversion was performed to check the promoter methylation that was quantified using the real time PCR. (B) Relative mRNA expression level of GATA3 in WT-FoxM1 or FoxM1 phospho-mutants expressing cells. (C) Relative mRNA expression level of cdc25b in the presence of WT-FoxM1 or its phospho-mutants. (D) Western blot showing expression of the wild type and mutant FoxM1 proteins. Statistical analysis was done using graph pad prism unpaired t test and p-values represented as *≤0.05; **≤0.01; ***≤0.001.

Back to article page