Figure 7: FoxM1b methylates and represses FoxA1 in MCF7 cells.

(A and B) MCF7 cells and MDA-MB-453 cells were transfected with Control siRNA or two different FoxM1siRNA and 72hr post transfected cells were harvested and RNA was used to quantify the FoxA1 transcripts using qRT- PCR. Silencing of FoxM1 expression in two cell lines was verified by western blots (lower panel). (C) Cells were transfected with empty vector control, WT-T7-FoxM1, T7-FoxM1 (596 A), T7-FoxM1 (DD) and T7-FoxM1 (AA) expressing plasmids. RNA was extracted 48hr post transfection for FoxA1 transcripts and quantification was performed by qRT-PCR. (D) genomic-DNAs from the transfected cells were isolated and C/T conversion was performed to check the promoter methylation that was quantified using the real time PCR. Statistical analysis was done using Graph pad prism unpaired t test and p-values represented as *≤0.05; **≤0.01; ***≤0.001.