Figure 5: Catalytic site-binding drugs inhibit CoV cell infection.
From: Allosteric inhibition of aminopeptidase N functions related to tumor growth and virus infection
(a) Inhibition of TGEV infection by various pAPN-binding drugs. Relative survival of ST cells infected with TGEV at different m.o.i., treated with 50 μM ABS1-4, actinonin (Ac) or DMSO (Methods). See Supplementary Fig. S3b. Mean ± SEM (n = 3). (b) Inhibition of TGEV cell entry by ABS4. Virus RNA was quantified by qRT-PCR 6 h post-infection, in samples without inhibitor (−), with ABS4 as indicated (μM) or with TGEV RBD-Fc (40 μg/ml) as positive control (RBD). Background signal determined with uninfected BHK cells (BHK). Mean ± SEM (n = 3). (c) Inhibition of TGEV cell infection with increasing ABS4 concentrations. ST cell survival was determined as in a. Mean ± SEM (n = 4).
