Figure 3: RGC32 induced EMT phenotype marker alterations in colorectal cancer.
From: RGC32 induces epithelial-mesenchymal transition by activating the Smad/Sip1 signaling pathway in CRC
(A) The morphology of SW480 cells transfected with vector or RGC32 plasmid (RGC32) and SW620 cells transfected with scramble or RGC32 shRNA (shRGC32). (B) The expression of mesenchymal and epithelial markers in SW480 cells transfected with vector or RGC32 plasmid (RGC32) and SW620 cells transfected with scramble or RGC32 shRNA (shRGC32) was detected by western blotting. (C) Confocal microscopy of E-cadherin and vimentin staining in SW620 cells transfected with scramble or RGC32 shRNA(shRGC32). (D) Immunohistochemical staining of the EMT-related proteins E-cadherin and Vimentin in primary tumor tissues derived from scramble or RGC32-containing SW620 cells as indicated. Magnification: 400x; *P < 0.05 vs scramble.
