Figure 2: PIK3CA mutation altered intracellular aqueous metabolite abundance in MCF10A cells.
Analysis was performed by GC-MS and data from both U-13C6 glucose and U-13C5 glutamine tracer experiments are included. For each metabolite the ion signals from individual isotopomers were summed and the metabolite intensity dataset were normalised through median fold change normalisation55. The bar graphs represent the mean ± SEM. Seven separate biological experiments are represented and the metabolite features are ranked according to the magnitude of difference between the mutant PIK3CA and the wild type parental line, with positive change representing an increase in the mutant compared to wild type cells. Multiple mass ion fragments may be detected for some metabolites; in those cases the m/z values of the distinct fragments are given e.g. lactate 261 (see Table S1). G3P (glycerol-3 phosphate) fragments are eluted at two separate retention times; *pairwise Student’s t-test p values < 0.05; **pairwise Student’s t-test p values < 0.005.
