Figure 3: Characterization of LSA3 in blood stage parasites. | Scientific Reports

Figure 3: Characterization of LSA3 in blood stage parasites.

From: Immunoscreening of Plasmodium falciparum proteins expressed in a wheat germ cell-free system reveals a novel malaria vaccine candidate

Figure 3

(a) Human anti-LSA3-C-specific IgG has significant GIA activity. Human anti-LSA3-C specific IgG was tested at 0.48 mg/ml. Rabbit anti-His-GST IgG was tested at 20 mg/ml as a negative control. Three independent GIA experiments in triplicate were performed, and the mean and SEM are shown. (b) LSA3 was expressed in blood-stage parasites. Western blot was performed with human, mouse or rabbit anti-LSA3-C antibodies. Parasite lysate was obtained from 1 × 104 P. falciparum infected red cells at mixed developmental stages. Molecular weight marker (Lane 1), purified 0.5 ng of C-terminal His-tagged recombinant LSA3-C protein (Lane 2) and the blood-stage parasite lysate (Lane 3) were separated by SDS-PAGE under reducing conditions. (c) IFA of schizont-stage parasites. Human anti-LSA3-C antibody was used for the IFA. Mouse anti-AMA1, -RAP1, -RON2, or -RESA antibodies were used for counter-staining to determine subcellular localization of microneme, rhoptry body, rhoptry neck or dense granule, respectively. Parasite nuclei were stained with DAPI. Scale bars indicate 5 μm.

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