Figure 7: Physical interaction between SBNO1 and the FACT complex. | Scientific Reports

Figure 7: Physical interaction between SBNO1 and the FACT complex.

From: Notch and Hippo signaling converge on Strawberry Notch 1 (Sbno1) to synergistically activate Cdx2 during specification of the trophectoderm

Figure 7

(a) Interaction between SBNO1 and Ssrp1 was analyzed by co-immunoprecipitation. GFP-SBNO1 and Myc-Ssrp1 were expressed in 293 T cells and immunoprecipitated with indicated antibodies (α-GFP and α-Myc, respectively). GFP-SBNO1 was co-precipitated along Myc-Ssrp1 and vice versa. (b) SBNO1-V5, HA-Yap, FLAG-Tead4, Myc-Ssrp1 and/or Spt16-VSVG were co-expressed in 293 T cells. Yap, Tead4, Ssrp1 and Spt16 were co-precipitated with SBNO1, indicating formation of a complex. (c) When HA-Yap was precipitated, co-purified bands of Myc-Ssrp1 and Spt16-VSVG were faint (α-HA). Both Myc-Ssrp1 and Spt16-VSVG were co-precipitated with FLAG-Tead4, yet in the presence of HA-Yap, bands corresponding to Myc-Ssrp1 and Spt16-VSVG became faint (red arrowheads), indicating weak interaction between Tead4 and the FACT complex (α-FLAG). Uncropped image of blots are shown in Supplementary Fig. 12.

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