Figure 4: Effects of MT-6 on Δψm and Bcl-2 family proteins in ovarian cancer cells.
From: The apoptotic mechanisms of MT-6, a mitotic arrest inducer, in human ovarian cancer cells
(A,B) MT-6 induces Δψm loss in a time-dependent manner. Cells were treated with 0.3 μM MT-6 for different durations, and Δψm was (A) measured by flow cytometry following rhodamine 123 staining (10 μM) and (B) expressed as percentage loss compared with control cells. (C) MT-6 downregulates pro-survival Bcl-2 family proteins. SKOV3 cells were treated with different concentrations of MT-6. After incubation for 24 or 48 h, whole-cell lysates were analyzed by Western blotting. Similar results were obtained in at least three independent experiments.
