Figure 6: MT-6–induced Δψm loss and mitotic arrest are JNK dependent.
From: The apoptotic mechanisms of MT-6, a mitotic arrest inducer, in human ovarian cancer cells
(A,B) MT-6 upregulates p-JNK levels in a concentration- (A) and time- (B) dependent manner. Effects of MT-6 on survival signaling were investigated by Western blot analysis. (C) MT-6–induced mitotic arrest was reversed by cotreatment with the JNK inhibitor SP600125. SKOV3 cells were treated with the indicated concentrations of MT-6 for the indicated times, with or without SP600125 cotreatment, then whole-cell lysates were analyzed by Western blotting. (D) Cotreatment with the JNK inhibitor SP600125 substantially attenuates MT-6–induced Δψm loss. Cells were treated with 0.3 μM MT-6 for 24 h, then Δψm was measured by rhodamine 123 (10 μM) staining followed by flow cytometry analysis.
