Figure 4: Proximity ligation assays for protein interaction between AE1 and stomatin (AE1-stom).
From: Stomatin modulates the activity of the Anion Exchanger 1 (AE1, SLC4A1)
RBCs from a control (stomatin-positive) and an OHSt (stomatin-deficient) patient were treated as described in experimental procedures, using anti-AE1 along with anti-stomatin antibodies and anti-AE1 along with anti-ankyrin R antibodies. For controls, two other pairs of antibodies were used: anti-AQP1 along with anti-CAII (positive controls) and anti-AE1 along with anti-AQP1 (negative controls). After PLA, RBCs were examined by confocal microscopy using a Zeiss LSM700 inverted confocal microscope equipped with a x100 oil-immersion objective, numerical aperture 1.4. Z-stack confocal image capture was performed and analyzed using the ZEN software.
