Figure 5
From: Stomatin modulates the activity of the Anion Exchanger 1 (AE1, SLC4A1)
Expression of recombinant AE1 (a) and stomatin (b) in transfected HEK 293 cells. Recombinant AE1 was detected at the surface of the non-permeabilized cells by flow cytometry using mouse monoclonal BRIC6 antibody directed against AE1 extracellular domains. After cell fixation and permeabilisation, recombinant and/or endogenous stomatin was detected using goat polyclonal M-14 antibody. AE1− and AE1+ are non-induced or induced cells for AE1 expression, respectively. Stom + and stom ++ correspond to cells expressing endogenous and endogenous plus recombinant stomatin, respectively.
