Figure 6: Regulation of redox state by CH4.
5-d-old maize seedlings of ZD958 and ZJY1 were preincubated in the solution containing 0.65 mM CH4 for 1 d, and then transferred to half-strength Hoagland solutions with or without 20% PEG-6000 for 2 d. Afterwards, the contents of endogenous H2O2 (a) in root tissues was analyzed. Meanwhile, the contents of reduced ascorbic acid (AsA; b), dehydroascorbate (DHA; c), and AsA/DHA ratio (d) in root tissues were determined according to the method described by Law et al.51. Control seedlings were incubated in Hoagland solution alone. Data are presented as means ± SE (5 root parts per experiment performed three times). Bars with different letters denote significant differences according to multiple comparisons (P < 0.05).
