Table 1 Reduced and total AsA concentration.

From: Methane protects against polyethylene glycol-induced osmotic stress in maize by improving sugar and ascorbic acid metabolism

Treatment

Reduced AsA (nmol·g−1 FW)

Total AsA (nmol·g−1 FW)

ZD958

ZJY1

ZD958

ZJY1

Control

485.8 ± 32.6B

364.3 ± 39.4DE

563.7 ± 45.3bc

449.5 ± 41.4d

CH4

554.3 ± 27.5A

493.2 ± 45.1B

682.2 ± 29.2a

577.3 ± 30.8b

PEG

432.4 ± 37.9C

324.4 ± 29.8E

689.6 ± 30.3a

443.2 ± 32.1d

PEG + CH4

498.4 ± 46.3B

389.8 ± 37.7D

702.1 ± 38.9a

540.8 ± 43.4c

  1. 5-d-old maize seedlings of ZD958 and ZJY1 were preincubated in the solution containing 0.65 mM CH4 for 1 d, and then transferred to half-strength Hoagland solutions with or without 20% PEG-6000 for 2 d. Afterwards, the contents of reduced and total ascorbic acid (AsA) in root tissues were determined by using ascorbate oxidase (AO) method reported by Ueda et al.52. Control seedlings were incubated in Hoagland solution alone. Data are presented as means ± SE (5 root parts per experiment performed three times). Within each set of experiments, bars with different letters denote significant differences according to multiple comparisons (P < 0.05).
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