Figure 2: apterous neurons located in the brain are responsible for the mating phenotype.
From: apterous Brain Neurons Control Receptivity to Male Courtship in Drosophila Melanogaster Females
(a) apGAL4/Otd-nls:FLPo intersecting neurons labelled in 3 to 4 day old females. The reproductive system was dissected from females that also carried a tub-GAL80ts transgene; flies were incubated at 30 °C for 24 h prior to dissection. Intersecting neurons were visualized with anti-GFP (green) and the tissue counterstained with nc82 or phalloidin (magenta). Scale bar represents 50 μm for the Brain and VNC and 100 μm for the reproductive system. (b) Schematic depicting the intersectional strategy employed and quantification of mating of virgin females with silenced central brain apterous neurons. Genotypes shown correspond to those of virgin females. n values shown in parentheses. n.s., not significant, ****p < 0.0001, Fisher’s exact test.
