Figure 2: Comparison of uFBA and FBA reaction flux states.

(a) Percentage of reactions with significantly different fluxes between the uFBA and FBA models. (b) The Spearman correlation between uFBA and FBA flux states indicates that differences in flux estimates were not due to uniform increases or decreases in fluxes but a reordering of reactions from high to low flux. The null hypothesis is that the distribution of Spearman correlations is drawn from the same distribution when comparing candidate flux states from uFBA to uFBA or FBA to FBA. *p = 0.0; E. coli p = 0.995.