Figure 1: In vivo knockdown of nuclear HMGB1 expression in hepatocytes using siRNA. | Scientific Reports

Figure 1: In vivo knockdown of nuclear HMGB1 expression in hepatocytes using siRNA.

From: Down-regulation of nuclear HMGB1 reduces ischemia-induced HMGB1 translocation and release and protects against liver ischemia-reperfusion injury

Figure 1

Mice were injected intravenously with 50 μg of HMBG1-siRNA (si-HMGB1) or scrambled siRNA (Scramble), and liver tissues were harvested 48 h later. Liver tissues from healthy mice who received no treatment served as controls (Normal). (A) Western blot analysis for HMGB1 was performed using the total cellular protein in the liver tissues, with each lane representing a separate animal. The blots shown are representative of three experiments with similar results (a). The corresponding densitometric analyses are shown as bar graphs (b). The means ± SEM are reported (**P < 0.01, n = 3 per group). (B) Real-time PCR was performed to measure mRNA expression of HMGB1 (**P < 0.01, n = 6 per group). (C) In situ immunohistochemical staining showed much weaker positive staining of HMGB1 in the nucleus of hepatocytes in HMGB1-siRNA-treated mice than in normal untreated or scrambled siRNA- treated mice (magnification, x1000).

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