Figure 3: Down-regulation of HMGB1 attenuates I/R-induced pathologic damage. | Scientific Reports

Figure 3: Down-regulation of HMGB1 attenuates I/R-induced pathologic damage.

From: Down-regulation of nuclear HMGB1 reduces ischemia-induced HMGB1 translocation and release and protects against liver ischemia-reperfusion injury

Figure 3

Mouse livers were subjected to 60 min of ischemia, followed by 6 h of reperfusion. The livers were harvested from the unclamped mice (sham), or mice clamped and pretreated with PBS, scrambled siRNA (scramble), or HMGB1-siRNA (si-HMGB1). (A) Hematoxylin and eosin (H&E) staining was performed to evaluate the pathological changes associated with liver damage (original magnification, x200); in situ TUNEL assays were performed to assess hepatic cell apoptosis (original magnification, x400); MPO assays were performed to evaluate liver neutrophil infiltration (original magnification, x400). Images are representative liver sections from 9 mice per group. (B) Quantitative assessment of liver necrosis (a), apoptotic cells (b), and MPO-positive cells (c), performed as described in Methods (**P < 0.01, n = 9 per group). The data shown are means ± SEM.

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