Figure 6: Determination of hydrogen sulfide in human blood (n = 6).

(a) The baseline hydrogen sulfide in human blood, plasma, and red cells. (b) The hydrogen sulfide levels in human blood, plasma, and red cells pretreated with DTT. (c) The DTT reducible hydrogen sulfide levels in human blood, plasma, and red cells. (d) The ratio between calculated hydrogen sulfide and determined hydrogen sulfide levels in human blood with or without DTT. (e) The protein binding rate of hydrogen sulfide in human plasma pretreated with or without DTT. (f) The typical blank subtracted sensorgrams of hydrogen sulfide to human serum protein (HSA) with the hydrogen sulfide levels ranging from 0 to 10 mM. (g) The typical blank subtracted sensorgrams of hydrogen sulfide to human hemoglobin (HB) with the hydrogen sulfide levels ranging from 0 to 10 μM. Data are represented as the mean ± SD, except for the sensorgrams. (h) The blank subtracted sensorgrams of (S)-warfarin to human serum protein (HSA) with hydrogen sulfide levels ranging from 0 to 64 nM.