Figure 1: Seasonal variations in eDNA concentration, in relation to adult and larval population size, adult body condition and temperature.
From: Seasonal variation in environmental DNA in relation to population size and environmental factors

(a) Shows Log10 (x + 0.0001) of the mean eDNA concentration (ngμL−1), per pond (black line, solid circles collected using glass-microfiber filters, solid squares collected using precipitation in ethanol) with 95% confidence intervals (grey) across the eight ponds. (b) Shows the mean estimated population size per pond black (adults - solid line, larvae - broken line) with 95% confidence intervals (grey). (c) Shows mean body condition (males – solid line, females – dashed line) using the scaled mass index of adults caught each week throughout a survey season with 95% confidence intervals (grey). (d) Shows mean weekly temperatures in degrees Celsius through the study period. The vertical dotted line represents the end of the breeding season and the start of the post-breeding season, as related to the models described in Tables 1, 2.