Figure 4: Cell proliferation was assessed immunocytochemically using the EdU incorporation assay immediately after anesthesia. | Scientific Reports

Figure 4: Cell proliferation was assessed immunocytochemically using the EdU incorporation assay immediately after anesthesia.

From: Sevoflurane decreases self-renewal capacity and causes c-Jun N-terminal kinase–mediated damage of rat fetal neural stem cells

Figure 4

(a) Immunofluorescence images were obtained by the EdU incorporation (green, Alexa Fluor 488) assay, DAPI (blue) nuclear staining for EdU-positive cells, and Alexa Fluor 555-stained (pink, Alexa Fluor 555) for nestin-marked cells and then the images were merged. (b) Immunofluorescence images from FNSCs exposed to 4.8% sevoflurane for 6 h. (c) Statistical results of EdU-positive cell ratio in FNSCs exposed to different concentrations of sevoflurane (1.2%, 2.4%, and 4.8%) for 6 h. Data were obtained from at least three separate cultures, ***P < 0.001 versus control group.

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