Figure 1: Schematic diagram of the methodology used to obtain protein lists from HEY Oct4A KD samples. | Scientific Reports

Figure 1: Schematic diagram of the methodology used to obtain protein lists from HEY Oct4A KD samples.

From: Knockdown of stem cell regulator Oct4A in ovarian cancer reveals cellular reprogramming associated with key regulators of cytoskeleton-extracellular matrix remodelling

Figure 1

HEY vector control and HEY Oct4A KD cells were prepared as whole cell lysate (n = 4), secretome (n = 4) and xenograft tumor samples (n = 4). Samples were solubilised, separated by short-range SDS-PAGE and subjected to in-gel reduction, alkylation, and tryptic digestion. Extracted peptides were fractionated and identified using mass spectrometry analysis, data processing database searching, informatics and protein annotation. Relative protein abundance was determined by estimating the ratio of normalised spectral counts (Rsc) between HEY Oct4A KD samples and HEY vector control samples for each protein. To determine the classification of proteins in response to Oct4A we applied a stringent analysis filtering criteria. The number of proteins which met the selection criteria for each HEY Oct4A KD sample group is listed.

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