Figure 5

(a) Expression of Oct4A, TUBB2A, PLEC and VIM in HEY vector control and Oct4A KD cells. RNA from HEY vector control and HEY Oct4A KD cells was extracted, cDNA was prepared and RT-PCR was performed as described in the Materials and Methods. The resultant mRNA levels were normalized to 18S mRNA. Results are representation of three independent experiments performed in triplicate. Significant variations between vector control and Oct4A KD cells was analysed by unpaired t-test with Welch’s correction using GraphPad Prism 7.02 and are indicated by *P < 0.05. (b) Expression of Oct4, TUBB2A, PLEC and VIM in mouse tumor xenografts generated by HEY vector control and Oct4A KD cells. Representative immunohistochemistry images of mouse xenografts for the expression of Oct4A, TUBB2A, PLEC and VIM. Images are set at 400x magnification and scale bar represents 60 μM (c) Variations in staining were determined by subtracting the negative control DAB readings number of strong positivity (Nsp)/area from the DAB readings of protein of interest for each xenograft. Data is presented as the mean ± SEM.