Figure 6: Expression of Oct4A, TUBB2A, PLEC and VIM in parental HEY, SKOV3 and OVCAR5 cell lines in response to paclitaxel or cisplatin treatment. | Scientific Reports

Figure 6: Expression of Oct4A, TUBB2A, PLEC and VIM in parental HEY, SKOV3 and OVCAR5 cell lines in response to paclitaxel or cisplatin treatment.

From: Knockdown of stem cell regulator Oct4A in ovarian cancer reveals cellular reprogramming associated with key regulators of cytoskeleton-extracellular matrix remodelling

Figure 6

(a) HEY, (b) SKOV3 and (c,d) OVCAR5 cell lines were treated with GI50 concentrations of paclitaxel or cisplatin for 72 hrs. RNA with and without paclitaxel or cisplatin treatments was extracted, cDNA was prepared and RT-PCR was performed as described in the Materials and Methods. The resultant mRNA levels were normalized to 18S mRNA. Results are representation of three independent experiments performed in triplicate. Significant variations between treated and untreated cells (control) was analysed by unpaired t-test with Welch’s correction using GraphPad Prism 7.02 and are indicated by *P < 0.05; **P < 0.01.

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