Table 5 Overview of the resistance mutations detected and interpreted by Sanger sequencing compared with WGS tools.

From: Mycobacterium tuberculosis resistance prediction and lineage classification from genome sequencing: comparison of automated analysis tools

  1. Black boxes show if a mutation was called and interpreted as causing resistance by a particular WGS analysis tool based on their respective mutation catalogues (Supplementary Table S3). For CASTB, the black boxes corresponded to a resistance call, which were included for completeness, although it was unclear whether the mutations in questions were actually the ones that were interpreted since the tool does not provide a mutation catalogue. The numbers in parentheses represent 95% confidence intervals. The equivalent table with Sanger sequencing as comparator can be found in Supplementary Table S4. 1Only a nucleotide change that resulted in a different amino acid change at that position was included in the resistance catalogue of this tool. 2It was unclear why this mutation, which featured in the resistance catalogue of this tool, was not reported. 3Reported as S171S (i.e. the mutation was translated despite the fact that it was within the 16S rRNA region).
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