Figure 1: MAOA expression and activity increases in androgen deprivation-induced NE differentiated LNCaP cells. | Scientific Reports

Figure 1: MAOA expression and activity increases in androgen deprivation-induced NE differentiated LNCaP cells.

From: MAOA-a novel decision maker of apoptosis and autophagy in hormone refractory neuroendocrine prostate cancer cells

Figure 1

(A) LNCaP, PC3, and DU145 were examined for REST and MAOA protein expression by immunoblotting (upper panel). GAPDH was used as control. MAOA mRNA was analyzed by RT-qPCR (lower panel). Data represent mean ± S.D. (n = 3); ***p < 0.001 by Student’s-t test. Uncropped images are presented in Supplementary Figure S9A. (B) Representative photos (50x magnification) of LNCaP cells cultured with 10% FBS, 10% or 2.5% charcoal/dextran-treated FBS (CDT) for 96 hours (upper panel). Average neurite elongation was quantified from 150 to 250 cells in random fields of three independent experiments (lower panel). **p < 0.01, ***p < 0.001 by Student’s-t test. (C) The expression of NE markers (left panel) and MAOA (right panel) in LNCaP cells treated as described in (A) was analyzed by RT-qPCR and normalized with β-actin. Similar results were obtained using 18 S rRNA as internal control. Data represent mean ± S.D. (n = 3); ***p < 0.001 by Student’s-t test. (D) Immunoblotting of NE marker β-tubulin III and MAOA in LNCaP cells treated as described in (B). β-actin was used as loading control. Uncropped images are presented in Supplementary Figure S9B. (E) MAOA activity was measured in LNCaP cells treated as described in (A) using an isotopic MAOA catalytic activity assay. Data represent mean ± S.D. (n = 3); *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s-t test.

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