Figure 3: Knockdown of MAOA suppresses androgen deprivation-induced NED. | Scientific Reports

Figure 3: Knockdown of MAOA suppresses androgen deprivation-induced NED.

From: MAOA-a novel decision maker of apoptosis and autophagy in hormone refractory neuroendocrine prostate cancer cells

Figure 3

(A) Immunoblotting of MAOA in shCtrl and shMAOA LNCaP cells. GAPDH was used as the loading control. Uncropped images are presented in Supplementary Figure S11A. (B) The mRNA level of MAOA in control and MAOA knockdown LNCaP cells was assessed by RT-qPCR and normalized with β-actin. ***p < 0.001 by Student’s-t test. (C) Representative photos (50x magnification) of LNCaP-shCtrl and -shMAOA cells treated with 10% or 2.5% CDT for 96 hours (left panel). Average neurite elongation was quantified as described in Fig. 1B (right panel). ***p < 0.001 by Student’s-t test. (D) The mRNA levels of NE markers in cells treated as in (B) were assessed using RT-qPCR and normalized against β-actin. Similar results were obtained using 18 S rRNA as internal control. Data represent mean ± S.D. (n = 3); *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s-t test. (E) Immunoblotting analysis of MAOA and β-tubulin III in cells treated as described in (B). β-actin was used as loading control. Uncropped images are presented in Supplementary Figure S11B.

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