Figure 4: Schematic showing the experimental setup for the cell culture system and collected cytokine detection, respectively.
From: A promising biodegradable magnesium alloy suitable for clinical vascular stent application
The cell culture and cytokine detection system are fabricated from polydimethylsiloxane (PDMS) with individual hole size of Ø 7 × 5 mm on top of the substrate and microarray anti-body glass. Antibody microarrays were manufactured using a pin-spotting technique for assaying a panel of proteins produced by macrophages/FBGCs. An immunosandwich-based assay was used to detect the secretion footprint of human macrophages/FBGCs seeded on metal substrates.
