Figure 5: In vitro macrophage fusion and inflammatory cytokine and chemokine detection.
From: A promising biodegradable magnesium alloy suitable for clinical vascular stent application
Immunofluorescence images showing the morphology of macrophages/FBGCs cultured on (a) JDBM-2 and (b) 316L stainless steel for 12 h, 72 h and 144 h (from left to right). The cells were fixed and stained for cytoskeleton (Pholloidin 532 nm: green) and nuclei (DRAQ5 650 nm: purple). Multinuclear giant cells (arrowheads) were observed on both 316L stainless steel and JDBM-2 substrates due to the foreign body reaction. Cytokine and chemokine production of macrophages/FBGCs cultured on JDBM-2 and 316L stainless steel for (c) 24 h and (d) 72 h were determined by ELISA.
