Figure 1: Effects of TFV and ADV on cell growth, proliferation and toxicity. | Scientific Reports

Figure 1: Effects of TFV and ADV on cell growth, proliferation and toxicity.

From: Tenofovir and adefovir down-regulate mitochondrial chaperone TRAP1 and succinate dehydrogenase subunit B to metabolically reprogram glucose metabolism and induce nephrotoxicity

Figure 1

(a) HK-2 cells were treated with TFV and ADV at various concentrations for 5 days. Phase-contrast and fluorescent images were observed. (b,c) The inhibition of cell proliferation was calculated by dividing the number of YOYO®-1 fluorescent objects (cytotoxicity index) by the total number of objects. (d,e) Dose- and time- dependent cytotoxicity of TFV and ADV after incubation of HK-2 cells and RTEC. Cell viability was determined by MTT assay. (f) HK-2 cells treated with 0, 300, 1000 μM of TFV and ADV for 5 days were stained with Annexin-V/PI for FACS-based quantification of apoptotic cells. (g) Quantification of apoptotic cells in HK-2 cells per group. All results are presented as means ± SEM (N = 3, *P < 0.05, ***P < 0.001 vs control).

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