Figure 4: Analysis of genes involved in mtDNA replication after TFV and ADV treatments. | Scientific Reports

Figure 4: Analysis of genes involved in mtDNA replication after TFV and ADV treatments.

From: Tenofovir and adefovir down-regulate mitochondrial chaperone TRAP1 and succinate dehydrogenase subunit B to metabolically reprogram glucose metabolism and induce nephrotoxicity

Figure 4

(a) Western blot analysis of proteins responsible for mtDNA replication: POLG, SSBP1, TWINKLE, TFAM with β-actin as internal control in whole cell lysates (20 μg protein) obtained from HK-2 cells treated with 1000 μM TFV and ADV. (b) Quantification of proteins/β-actin normalized to controls. (c) Western blot and (d) quantitative analysis for the levels of Polg, Ssbp1, Twinkle and Tfam in RTEC. (e) mtDNA levels by real-time PCR for the mtDNA encoded tRNA-Leu, COII, ATPase 6, D-loop and 16 S rRNA in HK-2 cells and (f) RTEC. Mitochondria DNA level/nuclear β-globin were normalized to control. The cropped blots are displayed and full-length blots are presented in Supplementary Fig. 2. Values are presented as means ± SEM (N = 3, *P < 0.05, **P < 0.01, ***P < 0.001 vs control).

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